SCGSR final report

Soils from northwest Alaska were homogenized and pre-incubated at -2 and -6 degrees Celsius for three months after which they were incubated at 2,4,6,8,10 degrees Celsius for one week. After the week long incubation soils were extracted using 0.5M K2SO4, and chloroform extracted to measure microbial biomass and nutrient concentrations. Sub-samples were also sent to PNNL for MPLEx (Methanol chloroform extraction) to provide more comprehensive analysis of the molecular composition of organic matter using FT-ICR, NMR, GC-MS and LC-MS techniques. Lipidomics were also performed to ascertain if there were any significant shifts in microbial biomass.

Respiration measurements were taken daily during the incubation using a Li-850 bench top respiration unit. Below are the respiration rates for each sample, as well as the calculates total C respired.

## [1] "Linear mixed effects model. JD2 is incubation day,Inc_temp is incubation temperature, pre_inc is the pre incubation temperature "

## [1] "ANOVA results comparing total respiration at the end of incubation.pre_inc is the pre incubation temperature, Inc_temp is the incubation temperature"

##             Df Sum Sq Mean Sq F value   Pr(>F)    
## pre_inc      1  10682   10682   22.84 5.51e-05 ***
## Inc_temp     1 132694  132694  283.75 7.53e-16 ***
## Residuals   27  12627     468                     
## ---
## Signif. codes:  0 '***' 0.001 '**' 0.01 '*' 0.05 '.' 0.1 ' ' 1

Soil K2SO4 extracts were utilized to measure ammonium, Nitrate, Total free primary amines, phosphate, Total reducing sugars. Below are the concentration data. An asterisks indicates a significant (p<= 0.05) difference in pre-incubation temperature.

Extraction ANOVA results nutrients and TRS

Soil K2SO4 extracts were utilized to measure ammonium, Nitrate, Total free primary amines, phosphate, Total reducing sugars. Below is the concentration data.An asterisks indicates a significant (p<= 0.05) difference in pre-incubation temperature.

Extraction ANOVA results microbial biomass

Below is the relative quantification of compounds identified by gas chromatography within the MPLEx extracts.Little to no variation was identified that corresponds to the more broad metrics above in the soil nutrient section. The majority of compounds measured were unidentified.